• Franca Ngozi Okoye, Department of Biochemistry, University of Port Harcourt, Rivers State. Nigeria


The quest for import substituton for improvement of dwindling Nigerian economy calls for
formulation of goods with local raw materials. This study was aimed at formulating animal feed
using a natural bentonite found in Nigeria and also determine the effects of the bentonite
formulated feed on some biochemical parameters of wistaralbino rats (Rattusrattus). A total of
45 rats with average weight of 100g were divided into 5 groups including the control group.
Four groups were fed with feed containing bentonite, while the control group was fed standard
commercial feed for 21 days. The rats were sacrificed from each group after week one, week
two, and week three respectively. The blood was obtained for the determination of alanine
transaminase (ALT) and aspartate transaminase (AST). The results indicated that the rats fed
with the natural bentonite formulated feed showed significant weight increase with the highest
weight increase observed on the third week of feeding. There was no significant change in the
level of ALT and AST indicating that there was no adverse effect on the rat’s liver. Thus, the
Nigerian natural bentonite had great potential for animal feed formulation. It was, therefore,
Recommended for utilization in the formulation of animal feed.


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